Aopep ELISA Kit for Rat(Rattus norvegicus) | EIAab.comAopep ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Aopep(Gene name) > Rat

Aopep (Gene name),

Aminopeptidase O (Protein name ), AMPO_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Aopep(Onpep);

Protein name:

Aminopeptidase O(AP-O);

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.

Subcellular Location:

Cytoplasm

Protein Attributes:

Sequence length:

816

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

816:

GHMDKASAQV | VTEMLF

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P69527

Pfam:

PF09127

KEGG:

rno:290963

UniGene:

Rn.229038

UniGene:

Rn.263

STRING:

10116.ENSRNOP00000023638

Uniprot:

P69527

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ELISA Kit for Rat AP-O

Cat.:

E12538h

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Packing:

96T

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ELISA Kit for Rat AP-O

Cat.:

E12538m

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Packing:

96T

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ELISA Kit for Rat AP-O

Cat.:

E12538r

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Packing:

96T

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CLIA Kit for Rat AP-O

Cat.:

U12538r

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat AP-O

Cat.:

U12538m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Rat AP-O

Cat.:

U12538h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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Polyclonal Antibody for Rat AP-O

Cat.:

P12538Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat AP-O

Protein for Rat AP-O

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of AMPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	96-105
Plasma	101	92-106

Recovery was determined by spiking various levels of AMPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	90-100
Plasma	96	91-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	95-111%	97-109%	94-110%
EDTA plasma(n=5)	93-108%	97-105%	95-107%	96-106%
heparin plasma(n=5)	96-104%	94-109%	98-105%	97-107%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-104%	91-98%	90-101%	92-98%
EDTA plasma(n=5)	90-103%	93-100%	88-105%	90-104%
heparin plasma(n=5)	92-102%	91-102%	92-101%	93-98%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Identification of human aminopeptidase O, a novel metalloprotease with structural similarity to aminopeptidase B and leukotriene A4 hydrolase."
Diaz-Perales A. , Quesada V. , Sanchez L.M. , Ugalde A.P. , Suarez M.F. , Fueyo A. , Lopez-Otin C.
J. Biol. Chem.280:14310-14317(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Lung.

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